Application

Research Sub CategoryNuclear Receptors

This Anti-NRF-1 Antibody, clone R157.1.3H3 is validated for use in Western Blotting, Chromatin Immunoprecipitation (ChIP) and ChIP-seq for the detection of NRF-1.

Research CategoryEpigenetics & Nuclear Function

Western Blotting Analysis: 1.0 µg/mL from a representative lot detected NRF-1 in 10 µg of SH-SY5Y and HeLa cell lysates.
Chromatin Immunoprecipitation (ChIP) Analysis: A representative lot detected NRF-1 chromatin DNA binding activity using K562 chromatin preparations (ENCODE Data Coordination Center (DCC) accession: ENCAB000BLM).
ChIP-seq Analysis: A representative lot detected NRF-1 DNA binding motifs by ChIP-seq analysis using K562 chromatin preparations (ENCODE Data Coordination Center (DCC) accession: ENCAB000BLM).

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Nuclear respiratory factor 1 (UniProt Q16656; also known as Alpha-pal, Alpha palindromic-binding protein, NRF-1) is encoded by the NRF1 (also known as ALPHA-PAL) gene (Gene ID 4899) in human. NRF-1 is a redox-responsive transcription factor that plays an anti-oxidant, pro-survival role in mitochondrial biogenesis process. NRF-1 is upregulated and activated/phosphorylated by Akt in response to oxidant exposure or increased cellular reactive oxygen species (ROS), resulting in NRF-1 nuclear translocation, where it binds to DNA as a homodimer and mediates the transcription of mitochondrial transcription factor A (mtTFA) that is needed for mitochondrial DNA transcription.

Immunogen

Recombinant protein corresponding to human NRF-1.

Other Notes

Concentration: Please refer to lot specific datasheet.

Physical form

Protein G Purified

Purified mouse monoclonal IgG2aκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Format: Purified

Quality

Evaluated by Western Blotting in Raji cell lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected NRF-1 in 10 µg of Raji cell lysate.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~58 kDa observed. Uncharacterized band(s) may appear in some lysates.